Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz's Comprehensive Virology 11: Regulation and Genetics Plant PDF

By Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)

ISBN-10: 1468427210

ISBN-13: 9781468427219

ISBN-10: 1468427237

ISBN-13: 9781468427233

The time turns out ripe for a serious compendium of that section of the organic universe we name viruses. Virology, as a technological know-how, having handed only in the near past via its descriptive section of naming and num­ bering, has most likely reached that level at which quite few new­ actually new-viruses can be found. caused by way of the highbrow probes and methods of molecular biology, genetics, biochemical cytology, and excessive solution microscopy and spectroscopy, the sphere has skilled a real details explosion. Few critical makes an attempt were made to chronicle those occasions. This entire sequence, that allows you to include a few 6000 pages in a complete of approximately 22 volumes, represents a dedication by means of a wide crew of energetic investigators to research, digest, and expostulate at the nice mass of information in terms of viruses, a lot of that's now amorphous and disjointed, and scattered all through a large literature. during this method, we are hoping to put the full box in point of view, and to boost an invalu­ capable reference and sourcebook for researchers and scholars in any respect degrees. This sequence is designed as a continuum that may be entered at any place, yet which additionally offers a logical development of constructing evidence and built-in concepts.

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Translation of AMV-S in a wheat germ extract without spermine gave only a small amount of a 3a-like protein. , 1976). The translation products of AMV 425 RNA 3 are more heterogeneous: a peak at the position of coat protein, a dominating peak of a 3a-like protein, and a slower-migrating band (Fig. 38). , to be published). The pattern was not significantly influenced by the presence of coat protein during the translation of RNA 3. , "' 0 u A ~ M , M o )( 0 u ~ ~ 8 AMV-RNA 4 products 3 "' til B AMV-RNA 3 products , 0 2 ~ a..

Clark et al. (1974) purified the supernatant enzyme by a polymer partition system followed by a DEAE Sephadex and a phosphocellulose column. From healthy leaves, only a negligible RNA replicase activity was obtained by the same purification procedure. The preparation obtained from infected leaves contained, besides an RNAdependent polymerase activity, a poly(C)-dependent activity. The two activities eluted at different salt concentrations from a DNA agarose column. The purified enzyme was unstable at ODC and at - 15 DC, but it could be stored in liquid nitrogen without loss of activity.

However, the modified BMV RNA was still capable of functioning as messenger. 1). This could indicate that the structure of BMV RNAs 3 and/or 4 has been altered in such a way that the affinities (or accessibilities) of the ribosomes for the different cistrons have been changed. A possible explanation for Chapter I 38 these findings could be that not only the ribose residue at the 3' end of the RNA was attacked but also that of the m 7 G of the cap at the 5' end. 6). , 1974). Tyrosylated BMV RNA was found to interact with a binary complex of wheat germ elongation factor 1 and [3H]GTP.

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Comprehensive Virology 11: Regulation and Genetics Plant Viruses by Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)

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