By Dr Desmond S. T. Nicholl
During this 3rd variation of his well known undergraduate-level textbook, Des Nicholl recognises sound seize of easy ideas is key in any advent to genetic engineering. for that reason, in addition to being completely up-to-date, the publication additionally keeps its specialize in the elemental ideas utilized in gene manipulation. The textual content is split into 3 sections: half I presents an creation to the correct uncomplicated molecular biology; half II, the tools used to govern genes; and half III, functions of the know-how. there's a new bankruptcy dedicated to the rising significance of bioinformatics as a different self-discipline. different extra positive factors contain textual content packing containers, which spotlight vital features of subject matters mentioned, and bankruptcy summaries, which come with goals and studying results. those, besides key be aware listings, notion maps and a word list, will allow scholars to tailor their examine to fit their very own studying kinds and eventually achieve a company grab of a subject matter that scholars characteristically locate tough.
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Extra info for An introduction to genetic engineering
10). This feature can be used to provide information about the sequence complexity of the DNA in question, since sequences that are present as multiple copies in the genome will renature faster than sequences that are present as single copies only. By performing this type of analysis, eukaryotic DNA can be shown to be composed of four different abundance classes. First, some DNA will form duplex structures almost instantly, because the denatured strands have regions such as inverted repeats or palindromes, which fold back on each other to give a hairpin loop structure.
6 Note: Gene sizes are given in kilobase pairs (kbp). The number of exons is shown, and the percentage of the gene that is represented by these exons is given in the final column. is carried out in the nucleus, where the introns are spliced out of the primary transcript. Further intranuclear modiﬁcation includes the addition of a ‘cap’ at the 5 terminus and a ‘tail’ of adenine residues at the 3 terminus. These modiﬁcations are part of what is known as RNA processing, and the end product is a fully functional mRNA that is ready for export to the cytoplasm for translation.
13:16 P1: SBT 09780521850063c03 CUUS128/Nicholl 978 0 521 85006 3 March 11, 2008 WORKING WITH NUCLEIC ACIDS Fig. 4 Labelling DNA by primer extension (oligolabelling). (a) DNA is denatured to give single-stranded molecules. (b) An oligonucleotide primer is then added to give a short double-stranded region with a free 3 -OH group. (c) The Klenow fragment of DNA polymerase I can then synthesise a copy of the template strand from the primer, incorporating [α-32 P]dNTP (solid circles) to produce a labelled molecule with a very high specific activity.
An introduction to genetic engineering by Dr Desmond S. T. Nicholl